Searchable abstracts of presentations at key conferences in endocrinology

ea0019p310 | Steroids | SFEBES2009

Simultaneous measurement of cortisol and cortisone in saliva by LC-MS/MS: application in basal and stimulated conditions

Perogamvros I , Owen L , Ray D , Trainer P , Keevil B

Salivary cortisol is an increasingly popular tool in endocrine, psychological and sports studies. Immunoassays used for its measurement are limited by cross-reactivity from related steroids, mainly cortisone, which is abundant in saliva.A method was developed for the simultaneous measurement of cortisol and cortisone (SalF and SalE respectively) in saliva using LC-MS/MS. 40 μl of extract was injected onto a C8 4×2 mm guard cartridge attached to...